SERVICES
Introduction to prokaryotic expression services
E. coli expression system is the most widely used and most economical prokaryotic protein expression system. E. coli cells have clear genetic background, high target gene expression level, short culture period, strong anti-pollution ability, easy to enlarge, and can be applied to The expression of various genera proteins is an important tool for the development of recombinant protein expression and biotechnology industrialization, especially for the production of small molecular proteins. Yishen Shenzhou has a large number of E. coli soluble protein expression and purification and protein refolding experience, with a variety of E. coli cell lines and expression vectors, to provide you with high quality prokaryotic protein expression services.
Prokaryotic expression service technology advantages
More than 10 years of experience in prokaryotic expression
Multiple protein purification and protein refolding techniques
High-throughput, large-scale protein prokaryotic expression production capacity
Provide a variety of expression vectors and E. coli cells for one-stop services from genes to purified proteins
Prokaryotic expression service content
| Service steps and content | cycle | Delivery content |
| Gene synthesis and codon optimization (optional) | 1-2 weeks | |
| Vector construction | 1-2 weeks | Sequencing report |
• Clone to expression vector • Plasmid sequencing • Large amount of plasmid preparation | ||
| Expression and purification | 2-3 weeks | • Feasibility report • If the expression is feasible and there are remaining samples after QC testing, provide 3mg sample |
• Transform the appropriate strain • Affinity purification (superscript) • Denatured renaturation (inclusion body) • SDS-PAGE and UV analysis | ||
| Massive expression and purification (optional) | 2-3 weeks | • Purified protein • SDS-PAGE report • CoA |
