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Voles TP ELISA Kit
Material Number:B244011
size： 96 wells
price： $416.00
Inventory Status： In stock
Unit： EA
This TP ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of TP in the sample, this TP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus TP concentration. The concentration of TP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
| Product Name | Voles TP ELISA Kit |
|---|---|
| Material Number | B244011 |
| Assay Length | 2-4.5 hours |
| Assay Type | Solid Phase Sandwich ELISA |
| Validated Species | Natural and recombinant Voles TP |
| Application | ELISA |
| Cross Reactivity | There is no detectable cross-reactivity. |
| Pack Size | 96wells/kit, with removable strips. |
| Sensitivity | 1.0 μg/mL |
|---|---|
| Assay Range | 20 μg/mL – 640 μg/mL |
| Sample Type | Cell Culture Supernates, Serum，plasma,Tissue Lysates |
| Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=10) | 110 | 93-120 |
| Serum (n=5) | 102 | 90-117 |
| Plasma (n=5) | 100 | 92-116 |
| Tissue Lysates (n=15) | 96 | 83-114 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 15 | 15 | 15 | 15 | 15 | 15 |
| CV% | 7.2 | 6.3 | 5.1 | 9.9 | 11.2 | 7.5 |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| Serum(n=5) | 87-102% | 86-103% | 87-96% | 90-103% |
| Plasma(n=5) | 86-101% | 82-102% | 88-97% | 85-97% |
| Tissue Lysates(n=5) | 83-98% | 85-98% | 82-101% | 83-96% |
| Cell culture Supernates(n=5) | 82-97% | 85-98% | 85-98% | 83-95% |
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature.
The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
For research use only, not for use in diagnostic procedures!
1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microtiter plate.
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