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Fish LTB4 ELISA Kit
Material Number:B290316
size： 96 wells
price： $416.00
Inventory Status： In stock
Unit： EA
This LTB4 ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of LTB4 in the sample, this LTB4 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus LTB4 concentration. The concentration of LTB4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
| Product Name | Fish LTB4 ELISA Kit |
|---|---|
| Material Number | B290316 |
| Assay Length | 2-4.5 hours |
| Assay Type | Solid Phase Sandwich ELISA |
| Validated Species | Natural and recombinant Fish LTB4 |
| Application | ELISA |
| Cross Reactivity | There is no detectable cross-reactivity. |
| Pack Size | 96wells/kit, with removable strips. |
| Sensitivity | 1.0 pg/ml |
|---|---|
| Assay Range | 31.25 pg/ml – 1000 pg/ml |
| Sample Type | Cell Culture Supernates, Serum，plasma,Tissue Lysates |
| Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=10) | 110 | 93-120 |
| Serum (n=5) | 102 | 90-117 |
| Plasma (n=5) | 100 | 92-116 |
| Tissue Lysates (n=15) | 96 | 83-114 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 15 | 15 | 15 | 15 | 15 | 15 |
| CV% | 7.2 | 6.3 | 5.1 | 9.9 | 11.2 | 7.5 |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature.
The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
For research use only, not for use in diagnostic procedures!
1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microtiter plate.
2. Add 50μl of Standard or Sample to the appropriate wells. Blank well doesn’t add anyting.
3. Add 100μl of Enzymeconjugate to standard wells and sample wells except the blank well, cover with an adhesive strip and incubate for 60 minutes at 37°C.
4. Wash the Microtiter Plate 4 times.
Manual Washing - Remove incubation mixture by aspirating contents of the plate into a sink or proper waste container. Using a squirt bottle, fill each well completely with Wash Solution (1X), then aspirate contents of the plate into a sink or proper waste container. Repeat this procedure for a total of four times. After final wash, invert plate, and blot dry by hitting plate onto absorbent paper or paper towels until no moisture appears. Note: Hold the sides of the plate frame firmly when washing the plate to assure that all strips remain securely in frame.
Automated Washing- Aspirate all wells, then wash plates four times using Wash Buffer (1X%2
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